Journal: Redox Biology

Article Title: A novel role of KEAP1/PGAM5 complex: ROS sensor for inducing mitophagy

doi: 10.1016/j.redox.2021.102186

Figure Lengend Snippet: PGAM5 interferes with PINK1 processing independently of its phosphatase activity. A. PGAM5 mutant and isoform lacking phosphatase activity induce Parkin translocation. PC6 cells were transfected with Parkin-EYFP and PGAM5 wt, PGAM5 F244D or short PGAM5 isoform (isoform 2, UniProt #Q96HS1-2). ****P < 0.0001, n = 6 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. B. PGAM5 mutant lacking PARL/OMA1 cleaving site does not induce Parkin translocation. PC6 cells were transfected with Parkin-EYFP and PGAM5 or PGAM5 S24F. ****P < 0.0001, n = 6 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. C. TOMM7 overexpression increases the fraction of full-length PGAM5 in HEK293 cells expressing PGAM5-flag and TOMM7-myc. D. TOMM7 overexpression protects partially against PGAM5 induced Parkin translocation. PC6 cells were transfected with Parkin-EYFP, PGAM5 wt or/and TOMM7. ****P < 0.0001, n = 9 dishes, 20 fields per dish, Welch's ANOVA followed by Dunnett's T3 multiple comparisons test. E. OMA1 and PARL protect partially against PGAM5 induced Parkin translocation. PC6 cells were transfected with Parkin-EYFP, PGAM5 wt and PARL or OMA1. ****P < 0.0001, n = 6 dishes, 20 fields per dish, One-way ANOVA followed by Sidak's multiple comparisons test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: PARL-FLAG , Addgene , Cat# 13639.

Techniques: Activity Assay, Mutagenesis, Translocation Assay, Transfection, Over Expression, Expressing

Journal: Redox Biology

Article Title: A novel role of KEAP1/PGAM5 complex: ROS sensor for inducing mitophagy

doi: 10.1016/j.redox.2021.102186

Figure Lengend Snippet:

Article Snippet: PARL-FLAG , Addgene , Cat# 13639.

Techniques: Recombinant, Lysis, Extraction, Blocking Assay, Isolation, DC Protein Assay, Caspase-Glo Assay, shRNA, Generated, Plasmid Preparation, Synthesized, Variant Assay

Journal: FEMS Yeast Research

Article Title: A toolkit for rapid CRISPR- Sp Cas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains

doi: 10.1093/femsyr/foy107

Figure Lengend Snippet: Strains used in this study.

Article Snippet: Similarly, the plasmids pUDR214 and pUDR418 were constructed with the pROS10 backbone ( URA3 ) (Addgene plasmid #107924; Mans et al. ) and insert fragment amplified with primers containing spacer sgRNA targeting HXT2 (9574) and HXT13 , HXT15 , HXT16 (9573) recognition sequences and insert fragment amplified with primers containing the spacer sgRNA targeting STL1 (primer 13616) respectively (Fig. ).

Techniques:

Journal: FEMS Yeast Research

Article Title: A toolkit for rapid CRISPR- Sp Cas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains

doi: 10.1093/femsyr/foy107

Figure Lengend Snippet: ( A ) Overview of the chromosomal localization of the deleted hexose transporters. Genes indicated with the same color were removed in the same deletion round. Red, first round; blue, second round; green, third round. ( B ) Deletion strategy. The scissors indicate the gene targeted by Sp Cas9 editing. The circled numbers indicate the sgRNA used to guide Sp Cas9 for editing.

Article Snippet: Similarly, the plasmids pUDR214 and pUDR418 were constructed with the pROS10 backbone ( URA3 ) (Addgene plasmid #107924; Mans et al. ) and insert fragment amplified with primers containing spacer sgRNA targeting HXT2 (9574) and HXT13 , HXT15 , HXT16 (9573) recognition sequences and insert fragment amplified with primers containing the spacer sgRNA targeting STL1 (primer 13616) respectively (Fig. ).

Techniques:

Journal: FEMS Yeast Research

Article Title: A toolkit for rapid CRISPR- Sp Cas9 assisted construction of hexose-transport-deficient Saccharomyces cerevisiae strains

doi: 10.1093/femsyr/foy107

Figure Lengend Snippet: Plasmids used in this study.

Article Snippet: Similarly, the plasmids pUDR214 and pUDR418 were constructed with the pROS10 backbone ( URA3 ) (Addgene plasmid #107924; Mans et al. ) and insert fragment amplified with primers containing spacer sgRNA targeting HXT2 (9574) and HXT13 , HXT15 , HXT16 (9573) recognition sequences and insert fragment amplified with primers containing the spacer sgRNA targeting STL1 (primer 13616) respectively (Fig. ).

Techniques: